In the past, animal and cell culture studies have shown the protective effect of apple constituents on colon cancer. Especially cloudy apple juices seem to show protective effects on colon cancer formation. Together with cooperating network partners, these active apple ingredients will be identified using 'structure activity guided' fractionation. This means, after fractionation of the apple extract using separation techniques such as counter current chromatography (CCC), size exclusion chromatography (Sephadex), normal phase HPLC und if necessary other techniques (RP-18, XAD, silica gel, e.g.) the network partners will use the fractions for activity testing in their cell culture assays. Finally active apple ingredients will be identified using spectroscopic methods (HPLC-PAD, HPLC-ELSD, HPLC-MS/MS, and HPLC-NMR coupling techniques). To increase the information about the active ingredients derived from cloudy apple juices fermentation studies will be performed to identify metabolization products of chemopreventive apple polyphenols. Stoma effluent of colostomy patients will be used for ex vivo fermentations of apple polyphenols to achieve information about colonic metabolization products. Structural elucidation will be performed using HPLC-PAD, HPLC-DAD, HPLC-MS/MS, HRGC-MS, and NMR analyses.
Period: 1.7.2005 – 31.6.2009
Contact
Prof. Dr. Peter Schreier, Dr. Elke Richling
Universität Würzburg
Lehrstuhl für Lebensmittelchemie
Am Hubland
97074 Würzburg
Phone +49(0)931 888-5481, -4662
Fax +49(0)931 888-5484
schreier@pzlc.uni-wuerzburg.de
richling@pzlc.uni-wuerzburg.de
1.1 Uni Würzburg
Industrial Partner VK Mühlen AG